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Brews & Views Bulletin Board Service * Brews and Views Archive 2005 * Archive through April 25, 2005 * Slave to the yeast < Previous Next >

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Chumley
Senior Member
Username: Chumley

Post Number: 3047
Registered: 02-2003
Posted on Tuesday, April 19, 2005 - 10:38 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

I was going to go fishing Friday, but when I went home at lunch today, I popped open the cover of my 6.5 gallon plastic fermentor, and saw that, after two and a half weeks, the yeast has finally started to subside on my biere de garde.

So that means I will have a big ole yeast cake of WY1338 European Ale yeast hungry. I was going to dump it, but the thought of all that yeast ready to go has convinced me to brew an alt Friday instead. I'm also contemplating a NutriSystem start of CL50 tonight so I can have a double batch day.

Anyone else a slave to their yeast's schedule, instead of their own?
 

Graham Cox
Member
Username: T2driver

Post Number: 104
Registered: 11-2004
Posted on Wednesday, April 20, 2005 - 12:02 am:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

To some extent. I don't brew unless the yeast are ready.

Enlighten us on the "NutriSystem start."
 

Chumley
Senior Member
Username: Chumley

Post Number: 3049
Registered: 02-2003
Posted on Wednesday, April 20, 2005 - 03:29 am:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

Oops...I meant "NutriStart"...it was a topic of discussion here a couple of weeks ago.

http://www.brewstersyeast.com/Products.asp?id=1

So I now see that these slants are some sort of solid (agar) that you can't just dump out of the tube...all this talk about sterilizing a loop becomes clear...so I guess I'll have to snag a big ole paper clip from work tomorrow, bend it around, and bake it in the oven (or soak in iodophor)...unless someone else has a better way?
 

Guy C
Member
Username: Ipaguy

Post Number: 221
Registered: 09-2003
Posted on Wednesday, April 20, 2005 - 03:48 am:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

My yeast consider me their bitch.
 

Joseph Listan
Advanced Member
Username: Poonstab

Post Number: 629
Registered: 03-2003
Posted on Wednesday, April 20, 2005 - 04:07 am:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

I've said it before: My yeast consider me their god. Only *I* have absolute power over their entire existence: their food, the temperature of their world, their reporductive cycle, their very lives!

Fear me, puny fungi!




Then I drink their piss.

I've drunk a good bit of it very recently, in fact...
 

Mike Huss
Advanced Member
Username: Mikhu

Post Number: 599
Registered: 03-2003
Posted on Wednesday, April 20, 2005 - 01:46 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

Chumley a paper clip will work, but if you have a hobby store in town they will have inoculation loops which just work a little better. They have a nice loop bent in to the end of the wire that is a much smaller gauge than a paper clip. Personally I also bought an alcohol lamp for sterilizing, they're cheap enough you might want to look into that if you stop to get a loop.
 

Aaron Meyer
Member
Username: Meyeaard

Post Number: 167
Registered: 11-2004
Posted on Wednesday, April 20, 2005 - 02:24 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

Hobby Store? What kind of hobby store has microbial propagation equipment?
 

Chumley
Senior Member
Username: Chumley

Post Number: 3050
Registered: 02-2003
Posted on Wednesday, April 20, 2005 - 02:37 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

My question exactly, Aaron. If I can go out and buy an inoculation loop I will, but a hobby store?

I opened up the yellow pages, and the only hobby stores listed sell models (railroads, cars, boats, planes). This kind of hobby store, Mike?
 

Mark Tigges
Intermediate Member
Username: Mtigges

Post Number: 276
Registered: 03-2003
Posted on Wednesday, April 20, 2005 - 03:03 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

Go to a lab supply store. Maybe phone your local Fisher and ask for a local supplier.

You can get one from B3 though.

However, IMO unless you're going to use the slant multiple times, or you are going to propogate on a plate there is a better way. Sterilize an eyedropper and use it to transfer 10ml or so of wort to the slant. Wait a couple of days, then pour this into your starter.

But, the best thing to do is to streak a plate from the slant, then innoculate 10ml of wort in a tube from the nicest smallest most circular colony on the plate.
 

Mike Huss
Advanced Member
Username: Mikhu

Post Number: 600
Registered: 03-2003
Posted on Wednesday, April 20, 2005 - 03:04 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

Yeah, exactly. One of our hobby stores that sells all that kind of stuff had them, that's where I bought mine. Most hobby stores sell chemistry kits and supplies, and loops and alcohol lamps fall under chemistry supplies, don't they?

Anyway, whatever category they fall under our hobby store had them. I also bought slant tubes and a rack there too, as well as petri dishes and stuff like that.
 

Chumley
Senior Member
Username: Chumley

Post Number: 3051
Registered: 02-2003
Posted on Wednesday, April 20, 2005 - 03:21 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

>>However, IMO unless you're going to use the slant multiple times, or you are going to propogate on a plate there is a better way. Sterilize an eyedropper and use it to transfer 10ml or so of wort to the slant. Wait a couple of days, then pour this into your starter.

Now that sounds like a plan. Thanks.

Guess I won't be brewing an APA this Friday with CL50 after all (was wanting to get a yeast cake ready for the DC RyePA). Oh well, I have a tube of WLP022 Essex that I need to use up - with Maris Otter, EKG and Fuggles at home, I think I'm in business.
 

John McElver
Member
Username: Johnmc

Post Number: 188
Registered: 02-2004
Posted on Wednesday, April 20, 2005 - 04:40 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

"But, the best thing to do is to streak a plate from the slant, then innoculate 10ml of wort in a tube from the nicest smallest most circular colony on the plate."

Mark,
Smallest and circular aren't the best criteria for colony evaluation. Assuming that a good streak for single colonies has been performed AND that the yeast sample is mostly composed of the yeast of interest instead of other microbes, the best colonies to pick are the ones that are the same approximate size and overall appearance (shape and color) as the vast majority of their neighbors. Usually this will be round, but some strains of yeast have rough margins on the colonies. Also, the smallest colony may be a petite or some other bad thing.
John
 

Vance Barnes
Senior Member
Username: Vancebarnes

Post Number: 1602
Registered: 03-2003
Posted on Wednesday, April 20, 2005 - 05:56 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

I'd go the easy route myself. Just use a propane torch to heat the end of your paper clip, touch that to a part of the ager with no yeast to cool it, and then just scrape a small amount of the yeast up with the end of the paper clip and put that into your NutriStart. With the amount of yeast it looks like are on their slants you should be able to get at least a dozen batches from the slant.

The slants I used to get from the YCK guy were probably tubes only 3-4" long and only about as big a diameter as a pen. I always ended up throwing them away because of their age before I'd use all the yeast on them.
 

Chumley
Senior Member
Username: Chumley

Post Number: 3055
Registered: 02-2003
Posted on Wednesday, April 20, 2005 - 08:29 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

>>a part of the ager with no yeast

umm...this may sound like a stupid question, but what part is that? The whole thing looks like homogeneous yellow wax to me.
 

Pete Mazurowski
Member
Username: Pete_maz

Post Number: 106
Registered: 07-2003
Posted on Wednesday, April 20, 2005 - 09:01 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

"with Maris Otter, EKG and Fuggles at home, I think I'm in business"

Chumley, you may want to lay off the Fuggles. I hear they taste like dirt.

(Message edited by pete maz on April 21, 2005)
 

Mark Tigges
Intermediate Member
Username: Mtigges

Post Number: 277
Registered: 03-2003
Posted on Wednesday, April 20, 2005 - 09:28 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

Chumley,

Just jab it through the yeast into the agar, you'll hear a little "sszzz" it'll be fine. In fact, just bringing it back up through the yeast will probably be sufficient to innoculate something, but digging around in the yeast for a bit will do. If you're going to streak a plate, you don't want too much, in fact, you don't even need to actually see them. But if you're going to innoculate 10ml of wort, you may as well try and scoop some up. And John up there, is exactly right. I was just being simple. I definitely should not have used the word smallest. The reason I used it though is you want to stay away from the big huge masses of colonies that grew into each other. At least that's what I've been told. It doesn't sound like you're streaking plates anyway.
 

Joseph Listan
Advanced Member
Username: Poonstab

Post Number: 631
Registered: 03-2003
Posted on Wednesday, April 20, 2005 - 11:08 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

>>>The reason I used it though is you want to stay away from the big huge masses of colonies that grew into each other.

Why?
 

John McElver
Member
Username: Johnmc

Post Number: 189
Registered: 02-2004
Posted on Thursday, April 21, 2005 - 12:56 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

Joseph,
Mark's on the money with staying away from the huge mass, because when you pick an individual colony, you know that you're picking the descendants of single yeast rather than a glob descended from many microorganisms. I used "microorganisms" on purpose, because there could be bacteria or other fungi in the big glob and you can't tell, because it's one blended mix. Most of the time, it's mostly the yeast you're after, but not always.
Streaking for single colonies (or diluting for single colonies) will give you an idea of what the yeast of interest looks like so you can pick with confidence.
HOWEVER, if you only pick one, you may pick one whose character has changed. I usually pick three to four and inoculate a separate 4-5 ml tube of starter with each. Then they get blended (after a quick sniff).
 

Mike Huss
Advanced Member
Username: Mikhu

Post Number: 613
Registered: 03-2003
Posted on Monday, April 25, 2005 - 05:25 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

So Chumley, did you find a loop?
 

Chumley
Senior Member
Username: Chumley

Post Number: 3090
Registered: 02-2003
Posted on Monday, April 25, 2005 - 05:55 pm:   Edit Post Delete Post View Post/Check IP    Ban Poster IP (Moderator/Admin only)

No...when I realized I wasn't going to get a starter of CL50 ready by Friday, I decided to split the yeast cake of the 1338 I already had, and used it on the APA. Then on Saturday I floated the Missouri River all day, and Sunday the stores were closed. So I'll try this again in a couple of weeks when my primaries are done.